1627580-64-6
Most orderedMOTS-c
A 16-residue mitochondrial-derived peptide encoded in mtDNA. Supplied lyophilised, released at ≥ 99.0% by RP-HPLC.
Typical release99.2%
From
£86.00
In stock
Mitochondrial & LongevityMost ordered
NAD+
β-Nicotinamide adenine dinucleotide · NAD · Nicotinamide adenine dinucleotide, oxidised form
The oxidised pyridine dinucleotide cofactor, supplied lyophilised for redox, sirtuin and PARP work. Released at ≥ 98.0% by HPLC.
Total, ex VAT
£42.00
- Released at ≥ 98.0% (HPLC, area %)
- Identity confirmed by ESI-MS against theoretical mass
- Batch certificate matched to the vial, verifiable online
- Same working day dispatch before 14:00, tracked
For laboratory research use only. Not for human or veterinary use, and not a medicine, food or cosmetic. Sold to qualified researchers and institutions who accept responsibility for safe handling and lawful use.
Compound data
What is in the vial
Indicative values for this line. The certificate issued with your batch is the authoritative record and reports the measured figures for the material you receive.
- CAS number
- 53-84-9
- Molecular formula
- C21H27N7O14P2
- Average mass
- 663.43 g/mol
- Class
- Pyridine dinucleotide redox cofactor
- Form as supplied
- Lyophilised powder, free acid
- Appearance
- White to very pale yellow hygroscopic powder
- Purity specification
- ≥ 98.0% (HPLC, area %)
- Solubility
- Freely soluble in water. Solutions are acidic and are markedly less stable above pH 7.
- Storage
- −20 °C, tightly sealed and desiccated. Strongly hygroscopic — equilibrate to room temperature before opening.
Targets and pathways
- Sirtuins (SIRT1–SIRT7) — deacylation cosubstrate
- Poly(ADP-ribose) polymerases (PARPs)
- CD38 / NAD+ glycohydrolases
- Oxidoreductase reactions as the NAD+/NADH couple
Overview
About NAD+
NAD+ is not a peptide. It is the oxidised form of nicotinamide adenine dinucleotide, a pyridine dinucleotide that sits at the centre of cellular metabolism in two distinct roles: as the hydride-accepting half of the NAD+/NADH redox couple, and as a consumed substrate for the sirtuin, PARP and CD38 enzyme families. We list it alongside the mitochondrial peptides because that second role is why most laboratories buying it are buying it.
The distinction matters analytically. Used as a redox cofactor, NAD+ is recycled and a working solution is comparatively forgiving. Used as a sirtuin or PARP cosubstrate, it is consumed stoichiometrically and cleaved to nicotinamide plus ADP-ribose — so cofactor depletion and nicotinamide product inhibition both become variables the experiment has to control for.
It is also one of the most demanding compounds in the catalogue to store correctly. The free acid is strongly hygroscopic and degrades in alkaline aqueous solution, hydrolysing to nicotinamide and ADP-ribose. A vial left open on a bench in a humid room will gain mass measurably within minutes, and that water is the start of the degradation. Ryzen supplies it desiccated and sealed, released at ≥ 98.0% by HPLC with water content reported by Karl Fischer on every certificate.
In short
- Free acid form, lyophilised, released at ≥ 98.0% area-percent by HPLC
- Water content determined by Karl Fischer titration and stated on every certificate
- Cosubstrate for sirtuin, PARP and CD38 assays as well as the NAD+/NADH redox couple
- Strongly hygroscopic — supplied desiccated with handling guidance
- Available in 100 mg, 500 mg and 1000 mg fills for method development through to routine assay
Where it is used
Research applications
Sirtuin enzymology
All seven sirtuins consume NAD+ as a cosubstrate for deacetylation and related deacylation reactions. Cofactor purity directly affects measured kinetics, and residual nicotinamide in a poor preparation inhibits the very reaction being measured.
PARP activity and DNA damage response
PARP enzymes consume NAD+ heavily during poly(ADP-ribosyl)ation following DNA strand breaks, making cofactor supply a limiting variable in damage-response models.
Redox biology
As the NAD+/NADH couple, the compound underpins the dehydrogenase assays used across intermediary metabolism, from glycolysis through to the TCA cycle.
Cellular ageing
Tissue NAD+ declines with age across several species, and the compound is central to work on precursor supplementation, CD38-mediated consumption and the salvage pathway.
Analytical standards
Purified NAD+ is used as a calibration standard in LC-MS quantitation of the nucleotide pool alongside NADH, NADP+ and NMN.
Handling
Reconstitution and stability
Reconstitution
Bring the sealed vial fully to room temperature before opening — opening a cold vial condenses atmospheric moisture directly onto a hygroscopic powder. Weigh quickly, reseal immediately and return to desiccated storage. Dissolve in water or a buffer at or below pH 7; NAD+ solutions are inherently acidic and are stable in that range.
Stability
Sealed, desiccated and held at −20 °C, the powder is stable for at least 24 months. In neutral aqueous solution at 2–8 °C, use within 7 days. Above pH 7 degradation is rapid and a solution should be regarded as single-session. Freeze aliquots at −80 °C where longer solution storage is unavoidable.
Notes
- Do not store working solutions in alkaline buffers. Hydrolysis to nicotinamide and ADP-ribose is base-catalysed and fast.
- Weigh in the shortest practical time. Mass gain from atmospheric water is the most common cause of a preparation reading low on concentration.
- A pale yellow tint in the powder is within specification. A brown tint is not — quote the batch code and we will replace it.
- Protect from light in solution; photodegradation contributes measurably over days.
Laboratory reference
Reconstitution calculator
Enter the fill weight on the vial and the diluent volume you intend to add. The calculator returns the resulting solution concentration and the volume that contains a given mass.
- Concentration
- 50.000 mg/mL · 50000 µg/mL
- Volume containing target mass
- 5.0 µL · 0.0050 mL
Figures are mass-to-volume arithmetic for laboratory preparation of NAD+ and take no account of net peptide content, which is stated on the batch certificate and should be applied to the fill weight before calculating. Nothing here constitutes dosing, administration or clinical guidance of any kind.
Published work
What the literature reports
Summaries of published findings, provided as research reference. These describe work done by others in preclinical and clinical settings; they are not claims about this material or outcomes for any reader.
NAD+ in ageing and metabolism
A widely cited review setting out the evidence for age-associated NAD+ decline, the enzymes that consume it, and the precursor strategies studied to restore tissue levels.
Rajman, Chwalek & Sinclair, Cell Metabolism, 2018
NAD+ metabolism in health and disease
A comprehensive treatment of biosynthesis via the salvage and de novo routes, compartmentalisation between cytosol, nucleus and mitochondria, and the consuming enzyme families.
Covarrubias et al., Nature Reviews Molecular Cell Biology, 2021
CD38 as a principal consumer
Work identifying CD38 as a major driver of age-related NAD+ decline established the enzyme as a target of interest and gave the field a mechanism for the decline rather than only an observation of it.
Camacho-Pereira et al., Cell Metabolism, 2016
Sirtuin dependence on cofactor availability
Foundational enzymology establishing that sirtuin deacetylation is NAD+-dependent and inhibited by nicotinamide, which remains the basis of most sirtuin assay design.
Imai et al., Nature, 2000
Questions
NAD+ — asked and answered
Something not covered here? Ask us directly — technical questions reach someone who runs the assays.
- Is NAD+ a peptide?
- No. It is a dinucleotide cofactor, not a peptide. We list it in the mitochondrial and longevity group because that is where most of the research demand for it sits, but it is chemically a different class and is assayed by a different HPLC method.
- Which form do you supply — free acid or disodium salt?
- Free acid, CAS 53-84-9. If your protocol calls for the disodium salt, contact us before ordering; the molecular weight differs and concentration calculations will not transfer directly.
- Why does the certificate report water content?
- Because NAD+ is strongly hygroscopic and water content directly affects the effective concentration of anything weighed from the vial. Karl Fischer titration gives the figure you need to correct for it. Certificates that omit water content on a hygroscopic compound leave you unable to calculate accurately.
- How long will a working solution last?
- Seven days at 2–8 °C at or below pH 7, protected from light. Above pH 7, treat it as single-session. Base-catalysed hydrolysis to nicotinamide and ADP-ribose is the limiting reaction.
- Do you supply NMN or NR as well?
- Not currently as catalogue lines. We quote on request for research quantities of the precursors, with the same release specification and certificate.
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Important
NAD+ is supplied strictly for laboratory research and analytical use. It is not a medicine, food, cosmetic or veterinary product; it is not for human or animal consumption; and it is not intended to diagnose, treat, cure or prevent any condition. It is not manufactured, tested or released to any standard that would support administration to humans or animals. Information on this page is compiled for research reference and is not medical advice. No dosing, administration or therapeutic guidance is given or implied. By ordering you confirm you are a qualified researcher or institution and accept responsibility for safe handling and lawful use.