1627580-64-6
Most orderedMOTS-c
A 16-residue mitochondrial-derived peptide encoded in mtDNA. Supplied lyophilised, released at ≥ 99.0% by RP-HPLC.
Typical release99.2%
From
£86.00
In stock
Mitochondrial & Longevity
Humanin
HN · Rattin (rodent homologue)
24-residue mitochondrial-derived peptide encoded in the 16S rRNA region of mtDNA.
Total, ex VAT
£126.00
- Released at ≥ 98.0% (RP-HPLC, area %)
- Identity confirmed by ESI-MS against theoretical mass
- Batch certificate matched to the vial, verifiable online
- Same working day dispatch before 14:00, tracked
For laboratory research use only. Not for human or veterinary use, and not a medicine, food or cosmetic. Sold to qualified researchers and institutions who accept responsibility for safe handling and lawful use.
Compound data
What is in the vial
Indicative values for this line. The certificate issued with your batch is the authoritative record and reports the measured figures for the material you receive.
- CAS number
- 330936-69-1
- Molecular formula
- C133H208N40O39S
- Average mass
- 2687.4 g/mol
- Sequence
- MAPRGFSCLLLLTSEIDLPVKRRA
- Residues
- 24
- Class
- Mitochondrial-derived peptide (MDP)
- Form as supplied
- Lyophilised powder
- Appearance
- White to off-white lyophilised powder
- Purity specification
- ≥ 98.0% (RP-HPLC, area %)
- Solubility
- Sparingly soluble in water; the central hydrophobic stretch promotes aggregation. DMSO stock followed by aqueous dilution is common practice.
- Storage
- Lyophilised: −20 °C, desiccated and dark. Reconstituted: 2–8 °C.
Targets and pathways
- Formyl peptide receptor-like 1 (FPRL1)
- IL-6 receptor family / gp130 signalling
- Bax-mediated apoptotic pathways
Overview
About Humanin
Humanin was the first mitochondrial-derived peptide identified, encoded within the 16S rRNA region of mitochondrial DNA. Like MOTS-c it demonstrated that mtDNA carries small open reading frames producing bioactive peptides, which reframed how the field thinks about mitochondrial signalling.
Handling is the practical challenge. The sequence contains a run of leucines that makes the peptide markedly hydrophobic and prone to aggregation in aqueous buffer. Most protocols prepare a DMSO stock and dilute into aqueous medium immediately before use; preparing directly into water frequently produces a suspension rather than a solution and a concentration that reads low.
In short
- The first mitochondrial-derived peptide identified, encoded in the mtDNA 16S rRNA region
- 24 residues with a hydrophobic central run — aggregation-prone
- DMSO stock and aqueous dilution is the usual preparation route
- Released at ≥ 98.0% area-percent by RP-HPLC
Where it is used
Research applications
Cytoprotection
The founding work identified humanin in a screen for protection against amyloid-beta toxicity, and cytoprotection remains its principal research use.
Mitochondrial signalling
Alongside MOTS-c, humanin is central to the study of mitochondrial-derived peptides as an outward signalling system.
Receptor pharmacology
Signalling through FPRL1 and gp130-family receptors is studied to establish how a mitochondrially encoded peptide reaches cell-surface receptors.
Handling
Reconstitution and stability
Reconstitution
Reconstitute with sterile or bacteriostatic water added slowly against the inner wall of the vial. Allow the cake to dissolve without agitation, swirling gently if required. Do not vortex or shake.
Stability
Lyophilised at −20 °C, desiccated and protected from light, stable for at least 24 months. Reconstituted and held at 2–8 °C, use within 28 days. Avoid repeated freeze-thaw.
Notes
- Bring the vial to room temperature before opening so atmospheric moisture does not condense onto cold powder.
- Record the reconstitution date on the vial; concentration drift between runs is usually evaporation, not synthesis variance.
- Aliquot at first reconstitution where the working programme needs more than a few withdrawals.
Laboratory reference
Reconstitution calculator
Enter the fill weight on the vial and the diluent volume you intend to add. The calculator returns the resulting solution concentration and the volume that contains a given mass.
- Concentration
- 2.500 mg/mL · 2500 µg/mL
- Volume containing target mass
- 100.0 µL · 0.1000 mL
Figures are mass-to-volume arithmetic for laboratory preparation of Humanin and take no account of net peptide content, which is stated on the batch certificate and should be applied to the fill weight before calculating. Nothing here constitutes dosing, administration or clinical guidance of any kind.
Published work
What the literature reports
Summaries of published findings, provided as research reference. These describe work done by others in preclinical and clinical settings; they are not claims about this material or outcomes for any reader.
Identification
Humanin was identified in a functional screen of a cDNA library from an unaffected brain region, selecting for protection against amyloid-beta-induced death.
Hashimoto et al., PNAS, 2001
Mitochondrial-derived peptide family
A review positioning humanin, MOTS-c and the SHLP series as a family and setting out the evidence for mitochondrial-encoded signalling.
Kim, Xiao et al., Trends in Endocrinology & Metabolism
Questions
Humanin — asked and answered
Something not covered here? Ask us directly — technical questions reach someone who runs the assays.
- Why will it not dissolve in water?
- The central leucine-rich stretch is strongly hydrophobic. Prepare a DMSO stock first, then dilute into aqueous buffer immediately before use. Going straight into water usually gives a suspension, and a concentration that reads low on assay.
- Which analogue do you supply?
- Wild-type human humanin, unmodified. The S14G analogue is a different, more potent variant and is quoted separately on request.
- Does it aggregate in storage?
- Not as the lyophilised powder. Aggregation is a solution-phase problem, which is why we recommend preparing fresh rather than holding aqueous stocks.
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Important
Humanin is supplied strictly for laboratory research and analytical use. It is not a medicine, food, cosmetic or veterinary product; it is not for human or animal consumption; and it is not intended to diagnose, treat, cure or prevent any condition. It is not manufactured, tested or released to any standard that would support administration to humans or animals. Information on this page is compiled for research reference and is not medical advice. No dosing, administration or therapeutic guidance is given or implied. By ordering you confirm you are a qualified researcher or institution and accept responsibility for safe handling and lawful use.